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RACE Kit
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RACE Kit
spectfication
RNA can be analyzed by cloning and sequencing after target region was amplified through RT-PCR. However, in essence, there are many difficulties in obtaining full-length cDNA clones from mRNA. RACE (Rapid-Amplification of cDNA Ends) methods, which is performed for cloning the 3 end or 5 end region based on the sequence information of cDNA region already obtained, is effective to overcome these difficulties. 3 RACE Kit is a kit designed to specifically amplify the region which contains 3-end of target mRNA utilizing 3-RACE procedure. The supplied Oligo dT-3 sites Adaptor Primer is specially designed for efficient cDNA synthesis from 3-end of poly (A)+ RNA. 5' RACE Kit is a core set designed to perform the 5-RACE procedure. This set achieves amplification of an unknown 5-end region of mRNA utilizing inverse PCR and allows efficient 5-RACE. This set is optimized for the use with M-MLV Reverse Transcriptase. All the reagents required for cDNA synthesis from RNA and for the subsequent circularization of the obtained cDNA are supplied in this set.
Kit Component | 3' RACE Kit | 5' RACE Kit |
M-MLV(200U/ul) | 10µl | 10µl |
5×M-MLV Buffer | 100µl | 100µl |
dNTPs(10mM) | 40µl | 40µl |
RNasin(40U/ul) | 10µl | 10µl |
Oligo-dT anchor Primer(20uM) | 10µl | 10µl |
PCR anchor Primer | 40µl | 40µl |
Taq Polymerase(5U/ul) | 25µl | 25µl |
10×PCR Buffer | 200µl | 200µl |
A tailing Enzyme(40U/ul) | - | 10µl |
dATP(20mM) | - | 10µl |
5×A tailing Buffer | - | 100µl |
RNase-free ddH2O | 1ml | 1ml |